A

Annexin

nnexin VV

檢檢測細胞凋亡

測細胞凋亡 ........................................................................................2

實驗原理................................................................................................................2

實驗用品................................................................................................................2

操作步驟................................................................................................................3

Annexin V Blocking ..............................................................................................5

凋

凋亡細胞的

亡細胞的 DDNA

NA 斷斷裂片段分析

裂片段分析 .............................................................................7

實驗原理................................................................................................................7

實驗用品................................................................................................................8

操作步驟................................................................................................................9

B

BrdU

rdU FFlow

low KKits

its 檢檢測細胞增殖

測細胞增殖..............................................................................12

實驗原理..............................................................................................................12

BrdU Flow Kits 試劑盒.......................................................................................12

結果分析..............................................................................................................17

流式儀器設置指南..............................................................................................18

線

線粒體膜電位變化檢測細胞凋亡

粒體膜電位變化檢測細胞凋亡..........................................................................22

實驗原理..............................................................................................................22

實驗用品..............................................................................................................22

樣本制備..............................................................................................................23

結果分析..............................................................................................................24

注意事項..............................................................................................................24

A

Active

ctive CCaspase-3

aspase-3 檢檢測細胞凋亡

測細胞凋亡 ...........................................................................26

實驗原理..............................................................................................................26

實驗步驟..............................................................................................................27

結果分析..............................................................................................................28